Fluorescenční charakterizace zlatem modifikovaného liposomu s antisense n-myc DNA připojené k magnetizovatelným částicím se zapouzdřenými protinádorovými léky (doxorubicin, ellipticin a etoposid)

摘要

Liposome-based drug delivery systems hold great potential for cancer therapy. The aimof this study was to design a nanodevice for targeted anchoring of liposomes (with and withoutcholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attachedto its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine andetoposide were prepared. They were further characterized by measuring their fluorescence intensity,whereas the encapsulation efficiency was estimated to be 16%. The hybridization process ofindividual oligonucleotides forming the nanoconstruct was investigated spectrophotometricallyand electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached tothe nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg•mL(-1),respectively. The study succeeded in demonstrating that liposomes are suitable for the transport ofanticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.